Tributyrin, an oral butyrate analogue, induces apoptosis through the activation of caspase-3

Kevin O. Clarke, Rena Feinman, Lawrence Harrison

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The purpose of this study was to investigate the anti-proliferative and pro-apoptotic effects of the butyrate analogues, tributyrin (TB) and phenylbutyrate (PB), in a colon cancer model. We demonstrate that HT-29 colon cancer cells exposed to PB and TB result in growth inhibition associated with an induction of apoptosis mediated through the activation of caspase-3 activity. A block in the G1/S cell cycle traverse associated with a decrease in CDK2 (cyclin dependent kinase) protein levels and retinoblastoma protein hypophosphorylation was also noted after PB and TB exposure. Importantly, TB proved to be the most potent agent in its ability to induce these phenotypic changes, and potentially may represent a novel therapy for patients with advanced colorectal cancer.

Original languageEnglish (US)
Pages (from-to)57-65
Number of pages9
JournalCancer Letters
Volume171
Issue number1
DOIs
StatePublished - Aug 28 2001
Externally publishedYes

Fingerprint

Phenylbutyrates
Butyrates
Caspase 3
Apoptosis
Colonic Neoplasms
Retinoblastoma Protein
Cyclin-Dependent Kinases
Colorectal Neoplasms
Cell Cycle
tributyrin
Growth
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

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abstract = "The purpose of this study was to investigate the anti-proliferative and pro-apoptotic effects of the butyrate analogues, tributyrin (TB) and phenylbutyrate (PB), in a colon cancer model. We demonstrate that HT-29 colon cancer cells exposed to PB and TB result in growth inhibition associated with an induction of apoptosis mediated through the activation of caspase-3 activity. A block in the G1/S cell cycle traverse associated with a decrease in CDK2 (cyclin dependent kinase) protein levels and retinoblastoma protein hypophosphorylation was also noted after PB and TB exposure. Importantly, TB proved to be the most potent agent in its ability to induce these phenotypic changes, and potentially may represent a novel therapy for patients with advanced colorectal cancer.",
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Tributyrin, an oral butyrate analogue, induces apoptosis through the activation of caspase-3. / Clarke, Kevin O.; Feinman, Rena; Harrison, Lawrence.

In: Cancer Letters, Vol. 171, No. 1, 28.08.2001, p. 57-65.

Research output: Contribution to journalArticle

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