The effect of glutamine- and alanine-enriched total parenteral nutrition on postburn proteolysis in the rat

Rolando Rolandelli, Mark J. Koruda Md, Steven G. Fukuchi, John L. Rombeau, T. Peter Stein

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study compared the effects of total parenteral nutrition (TPN) supplemented with either glutamine (GLN) or alanine (ALA) on postburn proteolysis in the rat. Thirty adult Sprague-Dawley rats underwent catheterization of the superior vena cava and placement of a swivel apparatus. One group (CONTROL, N = 18) was then returned to their cages while the remaining animals (BURN, N = 12) received a full-thickness scald burn (approximately 20% BSA). CONTROL and BURN rats were then randomly assigned to receive TPN supplemented with either GLN or ALA. TPN consisted of dextrose (250 Kcal/Kg BW/day) and crystalline amino acids (0.9 gN/kg BW/day) composed of all essential amino acids, histidine, arginine, glycine, and either ALA or GLN (1.2 g/100 mg). TPN was administered for 3 days after the burn. Urinary 3-methyl histidine (3MH) and nitrogen excretions were determined, and blood amino acids were measured. Both BURN groups had significantly higher 3MH excretion and blood levels of branched chain amino acids (BCAA) and alanine than both CONTROL groups. In both BURN and CONTROL groups, GLN-TPN did not significantly affect 3MH excretion or the blood levels of BCAA or alanine compared with ALA-TPN. In BURN animals, GLN-TPN resulted in significantly higher urinary nitrogen excretion than ALA-TPN resulting in lower nitrogen retention. Compared with ALA-TPN, GLN-TPN produced greater urinary nitrogen losses and did not reduce protein catabolism.

Original languageEnglish (US)
Pages (from-to)28-30
Number of pages3
JournalJournal of Nutritional Biochemistry
Volume9
Issue number1
DOIs
StatePublished - Jan 1 1998

Fingerprint

Proteolysis
Total Parenteral Nutrition
Nutrition
Glutamine
Alanine
Rats
Histidine
Nitrogen
Branched Chain Amino Acids
Blood
Animals
Amino Acids
Superior Vena Cava
Essential Amino Acids
Catheterization
Glycine
Sprague Dawley Rats
Arginine

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Rolandelli, Rolando ; Koruda Md, Mark J. ; Fukuchi, Steven G. ; Rombeau, John L. ; Stein, T. Peter. / The effect of glutamine- and alanine-enriched total parenteral nutrition on postburn proteolysis in the rat. In: Journal of Nutritional Biochemistry. 1998 ; Vol. 9, No. 1. pp. 28-30.
@article{cc231eb176a5496591412848130e3e72,
title = "The effect of glutamine- and alanine-enriched total parenteral nutrition on postburn proteolysis in the rat",
abstract = "This study compared the effects of total parenteral nutrition (TPN) supplemented with either glutamine (GLN) or alanine (ALA) on postburn proteolysis in the rat. Thirty adult Sprague-Dawley rats underwent catheterization of the superior vena cava and placement of a swivel apparatus. One group (CONTROL, N = 18) was then returned to their cages while the remaining animals (BURN, N = 12) received a full-thickness scald burn (approximately 20{\%} BSA). CONTROL and BURN rats were then randomly assigned to receive TPN supplemented with either GLN or ALA. TPN consisted of dextrose (250 Kcal/Kg BW/day) and crystalline amino acids (0.9 gN/kg BW/day) composed of all essential amino acids, histidine, arginine, glycine, and either ALA or GLN (1.2 g/100 mg). TPN was administered for 3 days after the burn. Urinary 3-methyl histidine (3MH) and nitrogen excretions were determined, and blood amino acids were measured. Both BURN groups had significantly higher 3MH excretion and blood levels of branched chain amino acids (BCAA) and alanine than both CONTROL groups. In both BURN and CONTROL groups, GLN-TPN did not significantly affect 3MH excretion or the blood levels of BCAA or alanine compared with ALA-TPN. In BURN animals, GLN-TPN resulted in significantly higher urinary nitrogen excretion than ALA-TPN resulting in lower nitrogen retention. Compared with ALA-TPN, GLN-TPN produced greater urinary nitrogen losses and did not reduce protein catabolism.",
author = "Rolando Rolandelli and {Koruda Md}, {Mark J.} and Fukuchi, {Steven G.} and Rombeau, {John L.} and Stein, {T. Peter}",
year = "1998",
month = "1",
day = "1",
doi = "10.1016/S0955-2863(97)00163-0",
language = "English (US)",
volume = "9",
pages = "28--30",
journal = "Journal of Nutritional Biochemistry",
issn = "0955-2863",
publisher = "Elsevier Inc.",
number = "1",

}

The effect of glutamine- and alanine-enriched total parenteral nutrition on postburn proteolysis in the rat. / Rolandelli, Rolando; Koruda Md, Mark J.; Fukuchi, Steven G.; Rombeau, John L.; Stein, T. Peter.

In: Journal of Nutritional Biochemistry, Vol. 9, No. 1, 01.01.1998, p. 28-30.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of glutamine- and alanine-enriched total parenteral nutrition on postburn proteolysis in the rat

AU - Rolandelli, Rolando

AU - Koruda Md, Mark J.

AU - Fukuchi, Steven G.

AU - Rombeau, John L.

AU - Stein, T. Peter

PY - 1998/1/1

Y1 - 1998/1/1

N2 - This study compared the effects of total parenteral nutrition (TPN) supplemented with either glutamine (GLN) or alanine (ALA) on postburn proteolysis in the rat. Thirty adult Sprague-Dawley rats underwent catheterization of the superior vena cava and placement of a swivel apparatus. One group (CONTROL, N = 18) was then returned to their cages while the remaining animals (BURN, N = 12) received a full-thickness scald burn (approximately 20% BSA). CONTROL and BURN rats were then randomly assigned to receive TPN supplemented with either GLN or ALA. TPN consisted of dextrose (250 Kcal/Kg BW/day) and crystalline amino acids (0.9 gN/kg BW/day) composed of all essential amino acids, histidine, arginine, glycine, and either ALA or GLN (1.2 g/100 mg). TPN was administered for 3 days after the burn. Urinary 3-methyl histidine (3MH) and nitrogen excretions were determined, and blood amino acids were measured. Both BURN groups had significantly higher 3MH excretion and blood levels of branched chain amino acids (BCAA) and alanine than both CONTROL groups. In both BURN and CONTROL groups, GLN-TPN did not significantly affect 3MH excretion or the blood levels of BCAA or alanine compared with ALA-TPN. In BURN animals, GLN-TPN resulted in significantly higher urinary nitrogen excretion than ALA-TPN resulting in lower nitrogen retention. Compared with ALA-TPN, GLN-TPN produced greater urinary nitrogen losses and did not reduce protein catabolism.

AB - This study compared the effects of total parenteral nutrition (TPN) supplemented with either glutamine (GLN) or alanine (ALA) on postburn proteolysis in the rat. Thirty adult Sprague-Dawley rats underwent catheterization of the superior vena cava and placement of a swivel apparatus. One group (CONTROL, N = 18) was then returned to their cages while the remaining animals (BURN, N = 12) received a full-thickness scald burn (approximately 20% BSA). CONTROL and BURN rats were then randomly assigned to receive TPN supplemented with either GLN or ALA. TPN consisted of dextrose (250 Kcal/Kg BW/day) and crystalline amino acids (0.9 gN/kg BW/day) composed of all essential amino acids, histidine, arginine, glycine, and either ALA or GLN (1.2 g/100 mg). TPN was administered for 3 days after the burn. Urinary 3-methyl histidine (3MH) and nitrogen excretions were determined, and blood amino acids were measured. Both BURN groups had significantly higher 3MH excretion and blood levels of branched chain amino acids (BCAA) and alanine than both CONTROL groups. In both BURN and CONTROL groups, GLN-TPN did not significantly affect 3MH excretion or the blood levels of BCAA or alanine compared with ALA-TPN. In BURN animals, GLN-TPN resulted in significantly higher urinary nitrogen excretion than ALA-TPN resulting in lower nitrogen retention. Compared with ALA-TPN, GLN-TPN produced greater urinary nitrogen losses and did not reduce protein catabolism.

UR - http://www.scopus.com/inward/record.url?scp=0031972979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031972979&partnerID=8YFLogxK

U2 - 10.1016/S0955-2863(97)00163-0

DO - 10.1016/S0955-2863(97)00163-0

M3 - Article

VL - 9

SP - 28

EP - 30

JO - Journal of Nutritional Biochemistry

JF - Journal of Nutritional Biochemistry

SN - 0955-2863

IS - 1

ER -