Temporal expression of TGF-β1, EGF, and PDGF-BB in a model of colonic wound healing

Michael A. Buckmire, Guido Parquet, Scott Greenway, Rolando Rolandelli

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Background. Dehiscence of colonic anastomoses is a multifactorial phenomenon. One mechanism by which this can occur is a deficiency of colonic submucosal collagen. Peptide growth factors (PGFs) have been shown to play a role in the synthesis, deposition, and maturation of collagen. Specifically, in tissues other than the colon, the transforming growth factor-beta (TGF- β1) gene has been shown to be temporally associated with expression of the procollagen gene. This study examines the temporal expression of the TGF- β1, epidermal growth factor (EGF), and platelet-derived growth factor B (PDGF-BB) genes and their temporal relationship to the expression of the procollagen type 1 (PROC I) gene. Materials and methods. Forty-eight Sprague- Dawley rats underwent transection of the descending colon with primary anastomosis. Perianastomotic colonic tissue was harvested on day 0 and postoperative days 3, 5, 6, 7, and 14. Colonic tissue was analyzed using semiquantitative reverse transcriptase-polymerase chain reaction and primers specific for the TGF-β1 EGF, and PDGF-B growth factors. Relative expression ratios of PGFs and PROC I genes were calculated versus a constitutive gene. Results. The data show that although all three of the PGFs genes were expressed in healing postoperative colonic tissue, only TGF-β1 showed a significant increase in its level of expression versus a constitutive gene from a mean ratio of 0.4 ± 0.08 on Day 0 to a mean ratio of 1.9 ± 0.27 on Day 7 (P < 0.0001 by ANOVA). The PROC I gene also showed a significant increase in expression (P < 0.001 by ANOVA) in the postoperative period which temporally correlated with the increase in the expression of the TGF-β1 gene (r = 0.89, P < 0.05). Conclusions. The temporal correlation between an increase in the gene expression of TGF-β1 and PROC I is initial evidence that that TGF-β1 plays a significant role in collagen metabolism in a healing colonic anastomosis.

Original languageEnglish (US)
Pages (from-to)52-57
Number of pages6
JournalJournal of Surgical Research
Volume80
Issue number1
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Epidermal Growth Factor
Wound Healing
Genes
Intercellular Signaling Peptides and Proteins
Collagen
Peptides
Analysis of Variance
Proto-Oncogene Proteins c-sis
Descending Colon
Gene Expression
platelet-derived growth factor BB
Procollagen
Collagen Type I
Reverse Transcriptase Polymerase Chain Reaction
Postoperative Period
Transforming Growth Factor beta
Sprague Dawley Rats
Colon

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Buckmire, Michael A. ; Parquet, Guido ; Greenway, Scott ; Rolandelli, Rolando. / Temporal expression of TGF-β1, EGF, and PDGF-BB in a model of colonic wound healing. In: Journal of Surgical Research. 1998 ; Vol. 80, No. 1. pp. 52-57.
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abstract = "Background. Dehiscence of colonic anastomoses is a multifactorial phenomenon. One mechanism by which this can occur is a deficiency of colonic submucosal collagen. Peptide growth factors (PGFs) have been shown to play a role in the synthesis, deposition, and maturation of collagen. Specifically, in tissues other than the colon, the transforming growth factor-beta (TGF- β1) gene has been shown to be temporally associated with expression of the procollagen gene. This study examines the temporal expression of the TGF- β1, epidermal growth factor (EGF), and platelet-derived growth factor B (PDGF-BB) genes and their temporal relationship to the expression of the procollagen type 1 (PROC I) gene. Materials and methods. Forty-eight Sprague- Dawley rats underwent transection of the descending colon with primary anastomosis. Perianastomotic colonic tissue was harvested on day 0 and postoperative days 3, 5, 6, 7, and 14. Colonic tissue was analyzed using semiquantitative reverse transcriptase-polymerase chain reaction and primers specific for the TGF-β1 EGF, and PDGF-B growth factors. Relative expression ratios of PGFs and PROC I genes were calculated versus a constitutive gene. Results. The data show that although all three of the PGFs genes were expressed in healing postoperative colonic tissue, only TGF-β1 showed a significant increase in its level of expression versus a constitutive gene from a mean ratio of 0.4 ± 0.08 on Day 0 to a mean ratio of 1.9 ± 0.27 on Day 7 (P < 0.0001 by ANOVA). The PROC I gene also showed a significant increase in expression (P < 0.001 by ANOVA) in the postoperative period which temporally correlated with the increase in the expression of the TGF-β1 gene (r = 0.89, P < 0.05). Conclusions. The temporal correlation between an increase in the gene expression of TGF-β1 and PROC I is initial evidence that that TGF-β1 plays a significant role in collagen metabolism in a healing colonic anastomosis.",
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Temporal expression of TGF-β1, EGF, and PDGF-BB in a model of colonic wound healing. / Buckmire, Michael A.; Parquet, Guido; Greenway, Scott; Rolandelli, Rolando.

In: Journal of Surgical Research, Vol. 80, No. 1, 01.01.1998, p. 52-57.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Temporal expression of TGF-β1, EGF, and PDGF-BB in a model of colonic wound healing

AU - Buckmire, Michael A.

AU - Parquet, Guido

AU - Greenway, Scott

AU - Rolandelli, Rolando

PY - 1998/1/1

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N2 - Background. Dehiscence of colonic anastomoses is a multifactorial phenomenon. One mechanism by which this can occur is a deficiency of colonic submucosal collagen. Peptide growth factors (PGFs) have been shown to play a role in the synthesis, deposition, and maturation of collagen. Specifically, in tissues other than the colon, the transforming growth factor-beta (TGF- β1) gene has been shown to be temporally associated with expression of the procollagen gene. This study examines the temporal expression of the TGF- β1, epidermal growth factor (EGF), and platelet-derived growth factor B (PDGF-BB) genes and their temporal relationship to the expression of the procollagen type 1 (PROC I) gene. Materials and methods. Forty-eight Sprague- Dawley rats underwent transection of the descending colon with primary anastomosis. Perianastomotic colonic tissue was harvested on day 0 and postoperative days 3, 5, 6, 7, and 14. Colonic tissue was analyzed using semiquantitative reverse transcriptase-polymerase chain reaction and primers specific for the TGF-β1 EGF, and PDGF-B growth factors. Relative expression ratios of PGFs and PROC I genes were calculated versus a constitutive gene. Results. The data show that although all three of the PGFs genes were expressed in healing postoperative colonic tissue, only TGF-β1 showed a significant increase in its level of expression versus a constitutive gene from a mean ratio of 0.4 ± 0.08 on Day 0 to a mean ratio of 1.9 ± 0.27 on Day 7 (P < 0.0001 by ANOVA). The PROC I gene also showed a significant increase in expression (P < 0.001 by ANOVA) in the postoperative period which temporally correlated with the increase in the expression of the TGF-β1 gene (r = 0.89, P < 0.05). Conclusions. The temporal correlation between an increase in the gene expression of TGF-β1 and PROC I is initial evidence that that TGF-β1 plays a significant role in collagen metabolism in a healing colonic anastomosis.

AB - Background. Dehiscence of colonic anastomoses is a multifactorial phenomenon. One mechanism by which this can occur is a deficiency of colonic submucosal collagen. Peptide growth factors (PGFs) have been shown to play a role in the synthesis, deposition, and maturation of collagen. Specifically, in tissues other than the colon, the transforming growth factor-beta (TGF- β1) gene has been shown to be temporally associated with expression of the procollagen gene. This study examines the temporal expression of the TGF- β1, epidermal growth factor (EGF), and platelet-derived growth factor B (PDGF-BB) genes and their temporal relationship to the expression of the procollagen type 1 (PROC I) gene. Materials and methods. Forty-eight Sprague- Dawley rats underwent transection of the descending colon with primary anastomosis. Perianastomotic colonic tissue was harvested on day 0 and postoperative days 3, 5, 6, 7, and 14. Colonic tissue was analyzed using semiquantitative reverse transcriptase-polymerase chain reaction and primers specific for the TGF-β1 EGF, and PDGF-B growth factors. Relative expression ratios of PGFs and PROC I genes were calculated versus a constitutive gene. Results. The data show that although all three of the PGFs genes were expressed in healing postoperative colonic tissue, only TGF-β1 showed a significant increase in its level of expression versus a constitutive gene from a mean ratio of 0.4 ± 0.08 on Day 0 to a mean ratio of 1.9 ± 0.27 on Day 7 (P < 0.0001 by ANOVA). The PROC I gene also showed a significant increase in expression (P < 0.001 by ANOVA) in the postoperative period which temporally correlated with the increase in the expression of the TGF-β1 gene (r = 0.89, P < 0.05). Conclusions. The temporal correlation between an increase in the gene expression of TGF-β1 and PROC I is initial evidence that that TGF-β1 plays a significant role in collagen metabolism in a healing colonic anastomosis.

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