Reoxygenation with 100% oxygen versus room air: Late neuroanatomical and neurofunctional outcome in neonatal mice with hypoxic-ischemic brain injury

Amy Presti, Sergei V. Kishkurno, Siarhei K. Slinko, Tara M. Randis, Veniamin I. Ratner, Richard A. Polin, Vadim S. Ten

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Study investigated neuroutcome in mice subjected at 7-8 d of life to hypoxic-ischemic brain injury (HI) followed by 30 min of reoxygenation with 100% O2 (Re-O2) or room air (Re-Air). At 24 h of recovery, mouse reflexes were tested. At 7 wks after HI spatial orientation and memory were assessed in the same mice. Mortality rate was recorded at 24 h and at 7 wks of recovery. In separate cohort of mice, changes in cerebral blood flow (CBF) during HI-insult and reoxygenation were recorded. Re-O2versus Re-Air mice exhibited significantly delayed geotaxis reflex. Adult Re-O2versus Re-Air mice exhibited significantly better spatial learning and orientation with strong tendency toward better preserved memory. Histopathology revealed significantly less hippocampal atrophy in Re-O2versus Re-Air mice. Following a hypoxia-induced hypoperfusion, Re-O2 re-established CBF in the ipsilateral side to the prehypoxic level significantly faster than Re-Air. The mortality was higher among Re-O2 versus Re-Air mice, although, it did not reach statistical significance. Re-O2versus Re-Air restores CBF significantly faster and results in better late neuroutcome. However, greater early motor deficit and higher mortality rate among Re-O2versus Re-Air mice suggest that Re-O2 may be deleterious at the early stage of recovery.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalPediatric Research
Volume60
Issue number1
DOIs
StatePublished - Jul 1 2006
Externally publishedYes

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Brain Injuries
Air
Oxygen
Cerebrovascular Circulation
Reflex
Mortality
Atrophy

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Presti, Amy ; Kishkurno, Sergei V. ; Slinko, Siarhei K. ; Randis, Tara M. ; Ratner, Veniamin I. ; Polin, Richard A. ; Ten, Vadim S. / Reoxygenation with 100% oxygen versus room air : Late neuroanatomical and neurofunctional outcome in neonatal mice with hypoxic-ischemic brain injury. In: Pediatric Research. 2006 ; Vol. 60, No. 1. pp. 55-59.
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Reoxygenation with 100% oxygen versus room air : Late neuroanatomical and neurofunctional outcome in neonatal mice with hypoxic-ischemic brain injury. / Presti, Amy; Kishkurno, Sergei V.; Slinko, Siarhei K.; Randis, Tara M.; Ratner, Veniamin I.; Polin, Richard A.; Ten, Vadim S.

In: Pediatric Research, Vol. 60, No. 1, 01.07.2006, p. 55-59.

Research output: Contribution to journalArticle

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T2 - Late neuroanatomical and neurofunctional outcome in neonatal mice with hypoxic-ischemic brain injury

AU - Presti, Amy

AU - Kishkurno, Sergei V.

AU - Slinko, Siarhei K.

AU - Randis, Tara M.

AU - Ratner, Veniamin I.

AU - Polin, Richard A.

AU - Ten, Vadim S.

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