Reduced Striatal [3H]Inositol 1,4,5‐Trisphosphate Binding in Huntington's Disease

Jerry J. Warsh, Jeffrey Politsky, Peter P. Li, Stephen J. Kish, O. Hornykiewicz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Abstract: Specific [3H]inositol 1,4,5‐trisphosphate ([3H]InsP3) binding was studied in regions of postmortem brain from 15 patients with Huntington's disease (HD) and 13 nonneurological controls. Single‐point binding analyses, using 5.0 nM InsP3, showed statistically significant reductions in specific [3H]InsP3 binding in the caudate (–71%) and putamen (–75%) of HD patients compared with controls. Frontal and occipital cortical [3H]InsP3 binding was not significantly different between HD and controls, a finding suggesting that the reduced [3H]InsP3 binding parallels the brain regional specificity of the neuropathological changes in HD. Scatchard analyses of data from [3H]InsP3 competition binding assays performed on caudate nucleus revealed that the reductions found using single‐point binding assays were due to a decrease in both binding density (–57%) and affinity (–50%) in HD brain compared with controls. The concomitant changes in InsP3 receptor density and affinity in HD brain suggest that these alterations may be produced by processes in addition to cell loss. These results suggest the possibility that disturbances in InsP3 receptor function, possibly resulting in altered intracellular calcium flux and homeostasis, occur in HD and may participate in the pathogenesis of this neurodegenerative disorder.

Original languageEnglish (US)
Pages (from-to)1417-1422
Number of pages6
JournalJournal of Neurochemistry
Volume56
Issue number4
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Corpus Striatum
Huntington Disease
Inositol
Brain
Assays
Caudate Nucleus
Putamen
Neurodegenerative Diseases
Homeostasis
Fluxes
Calcium

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Warsh, Jerry J. ; Politsky, Jeffrey ; Li, Peter P. ; Kish, Stephen J. ; Hornykiewicz, O. / Reduced Striatal [3H]Inositol 1,4,5‐Trisphosphate Binding in Huntington's Disease. In: Journal of Neurochemistry. 1991 ; Vol. 56, No. 4. pp. 1417-1422.
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Reduced Striatal [3H]Inositol 1,4,5‐Trisphosphate Binding in Huntington's Disease. / Warsh, Jerry J.; Politsky, Jeffrey; Li, Peter P.; Kish, Stephen J.; Hornykiewicz, O.

In: Journal of Neurochemistry, Vol. 56, No. 4, 01.01.1991, p. 1417-1422.

Research output: Contribution to journalArticle

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T1 - Reduced Striatal [3H]Inositol 1,4,5‐Trisphosphate Binding in Huntington's Disease

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AU - Hornykiewicz, O.

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