PCR detection of borrelia burgdorferi DNA in cerebrospinal fluid of lyme neuroborreliosis patients

Tracy E. Keller, John Halperin, Malcolm Whitman

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

We used the polymerase chain reaction (PCR), a method useful in the detection ofBorrelia burgdorferi in vitro to evaluate CSF in patients thought to have neuroborreliosis. Nested pairs of oligonucleotide primers were designed to recognize the C-terminalregion of B burgdorferi OspA. CSF samples were obtained from (1) patients with immunologic evidence of systemic B burgdorferi infection and clinical manifestations suggestiveof CNS dysfunction (2) seronegative patients with clinical disorders consistent with Lyme borreliosis and (3) patient and contamination controlsall were analyzed in a blinded fashion. PCRdetected B burgdorferi OspA DNA in CSF of (1)10 of 11 patients with Lyme encephalopathy (2) 28 of 37 patients with inflammatory CNS disease (3) seven of seven seronegative patients with Lyme-compatible disorders and (4) zero of 23 patient controls. Zero of 83 additional contamination controlswere PCR-positive. In eight patients from whom we obtained CSF before and after parenteral antimicrobial therapy PCR results invariably predicted clinical outcome accurately.

Original languageEnglish (US)
Pages (from-to)32-42
Number of pages11
JournalNeurology
Volume42
Issue number1
StatePublished - Jan 1 1992
Externally publishedYes

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Lyme Neuroborreliosis
Borrelia burgdorferi
Cerebrospinal Fluid
Polymerase Chain Reaction
DNA
Macrophage Colony-Stimulating Factor
DNA Primers
Lyme Disease
Central Nervous System Diseases
Brain Diseases

All Science Journal Classification (ASJC) codes

  • Clinical Neurology

Cite this

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PCR detection of borrelia burgdorferi DNA in cerebrospinal fluid of lyme neuroborreliosis patients. / Keller, Tracy E.; Halperin, John; Whitman, Malcolm.

In: Neurology, Vol. 42, No. 1, 01.01.1992, p. 32-42.

Research output: Contribution to journalArticle

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