Isolation and characterization of rabbit cardiac endothelial cells

Response to cyclic strain and growth factors in vitro

Mark Widmann, George V. Letsou, Stacie Phan, John C. Baldwin, Bauer E. Sumpio

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Studies of the cardiac endothelium have been complicated by difficulties in isolating and maintaining cardiac endothelial cells (EC) in culture. We present in this paper a method of obtaining pure EC from rabbit hearts by collagenase digestion and membrane filtration. Pure cultures of EC displaying characteristic EC morphology, uptake of di-I-acetylated LDL, and contact-inhibition of growth were successfully maintained in culture for several weeks in media supplemented with fetal bovine serum, bovine retina-derived growth factor (RDGF), and antibiotics. Since EC in vivo are exposed to a complex pattern of physical forces we also sought to determine the proliferative response of cardiac EC subjected to pulsatile strain in vitro and compared it with the response to the addition of an exogenous growth factor, RDGF. The results demonstrate that a regimen of 60 cycles per minute, 24% cyclic strain induced a significant increase in cardiac EC proliferation and suggests that physical forces may have a trophic effect on EC proliferation.

Original languageEnglish (US)
Pages (from-to)331-334
Number of pages4
JournalJournal of Surgical Research
Volume53
Issue number4
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Intercellular Signaling Peptides and Proteins
Endothelial Cells
Rabbits
Cell Proliferation
Contact Inhibition
In Vitro Techniques
Collagenases
Endothelium
Digestion
Cell Culture Techniques
Anti-Bacterial Agents
Membranes
Growth
Serum

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Widmann, Mark ; Letsou, George V. ; Phan, Stacie ; Baldwin, John C. ; Sumpio, Bauer E. / Isolation and characterization of rabbit cardiac endothelial cells : Response to cyclic strain and growth factors in vitro. In: Journal of Surgical Research. 1992 ; Vol. 53, No. 4. pp. 331-334.
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Isolation and characterization of rabbit cardiac endothelial cells : Response to cyclic strain and growth factors in vitro. / Widmann, Mark; Letsou, George V.; Phan, Stacie; Baldwin, John C.; Sumpio, Bauer E.

In: Journal of Surgical Research, Vol. 53, No. 4, 01.01.1992, p. 331-334.

Research output: Contribution to journalArticle

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