Intravenous butyrate and healing of colonic anastomoses in the rat

Rolando Rolandelli, Michael A. Buckmire, Keith A. Bernstein

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

PURPOSE: Intracolonic infusions of short chain fatty acids promote healing of colonic anastomoses. Because the intravenous route may have wider clinical application, we studied the effect of intravenous n-butyrate on the mechanical strength of colonic anastomoses in the rat. METHODS: After placement of an indwelling intravenous catheter, the descending colon was transected and an anastomosis was performed. Rats were then randomized to receive total parenteral nutrition (TPN group; n = 15) or total parenteral nutrition plus 130 mM/l of n-butyrate (TPN+BUT group; n = 13). On the fifth postoperative day, bursting pressure and bowel wall tension of the anastomoses were measured in situ. Anastomotic tissues were analyzed for hydroxyproline. RESULTS: The TPN+BUT group had a significantly higher bursting pressure (107.5 ± 30.3 vs. 83 ± 41.0 mmHg; P = 0.04) and bowel wall tension (20.7 ± 7.6 vs. 14.1 ± 9.9 Newton; P = 0.03). Tissue hydroxyproline was not different between the two groups (TPN, 45.8 ± 9.2, and TPN+BUT, 47.9 ± 2.9 μg/mg tissue nitrogen). CONCLUSIONS: We conclude that intravenous butyrate improves mechanical strength of a colonic anastomosis without a detectable change in total collagen content.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalDiseases of the Colon and Rectum
Volume40
Issue number1
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

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Butyrates
Total Parenteral Nutrition
Hydroxyproline
Descending Colon
Pressure
Indwelling Catheters
Volatile Fatty Acids
Nitrogen
Collagen

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Rolandelli, Rolando ; Buckmire, Michael A. ; Bernstein, Keith A. / Intravenous butyrate and healing of colonic anastomoses in the rat. In: Diseases of the Colon and Rectum. 1997 ; Vol. 40, No. 1. pp. 67-70.
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Intravenous butyrate and healing of colonic anastomoses in the rat. / Rolandelli, Rolando; Buckmire, Michael A.; Bernstein, Keith A.

In: Diseases of the Colon and Rectum, Vol. 40, No. 1, 01.01.1997, p. 67-70.

Research output: Contribution to journalArticle

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