Abstract
PURPOSE: Intracolonic infusions of short chain fatty acids promote healing of colonic anastomoses. Because the intravenous route may have wider clinical application, we studied the effect of intravenous n-butyrate on the mechanical strength of colonic anastomoses in the rat. METHODS: After placement of an indwelling intravenous catheter, the descending colon was transected and an anastomosis was performed. Rats were then randomized to receive total parenteral nutrition (TPN group; n = 15) or total parenteral nutrition plus 130 mM/l of n-butyrate (TPN+BUT group; n = 13). On the fifth postoperative day, bursting pressure and bowel wall tension of the anastomoses were measured in situ. Anastomotic tissues were analyzed for hydroxyproline. RESULTS: The TPN+BUT group had a significantly higher bursting pressure (107.5 ± 30.3 vs. 83 ± 41.0 mmHg; P = 0.04) and bowel wall tension (20.7 ± 7.6 vs. 14.1 ± 9.9 Newton; P = 0.03). Tissue hydroxyproline was not different between the two groups (TPN, 45.8 ± 9.2, and TPN+BUT, 47.9 ± 2.9 μg/mg tissue nitrogen). CONCLUSIONS: We conclude that intravenous butyrate improves mechanical strength of a colonic anastomosis without a detectable change in total collagen content.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 67-70 |
| Number of pages | 4 |
| Journal | Diseases of the Colon and Rectum |
| Volume | 40 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1997 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Gastroenterology
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Intravenous butyrate and healing of colonic anastomoses in the rat. / Rolandelli, Rolando; Buckmire, Michael A.; Bernstein, Keith A.
In: Diseases of the Colon and Rectum, Vol. 40, No. 1, 01.01.1997, p. 67-70.Research output: Contribution to journal › Article
TY - JOUR
T1 - Intravenous butyrate and healing of colonic anastomoses in the rat
AU - Rolandelli, Rolando
AU - Buckmire, Michael A.
AU - Bernstein, Keith A.
PY - 1997/1/1
Y1 - 1997/1/1
N2 - PURPOSE: Intracolonic infusions of short chain fatty acids promote healing of colonic anastomoses. Because the intravenous route may have wider clinical application, we studied the effect of intravenous n-butyrate on the mechanical strength of colonic anastomoses in the rat. METHODS: After placement of an indwelling intravenous catheter, the descending colon was transected and an anastomosis was performed. Rats were then randomized to receive total parenteral nutrition (TPN group; n = 15) or total parenteral nutrition plus 130 mM/l of n-butyrate (TPN+BUT group; n = 13). On the fifth postoperative day, bursting pressure and bowel wall tension of the anastomoses were measured in situ. Anastomotic tissues were analyzed for hydroxyproline. RESULTS: The TPN+BUT group had a significantly higher bursting pressure (107.5 ± 30.3 vs. 83 ± 41.0 mmHg; P = 0.04) and bowel wall tension (20.7 ± 7.6 vs. 14.1 ± 9.9 Newton; P = 0.03). Tissue hydroxyproline was not different between the two groups (TPN, 45.8 ± 9.2, and TPN+BUT, 47.9 ± 2.9 μg/mg tissue nitrogen). CONCLUSIONS: We conclude that intravenous butyrate improves mechanical strength of a colonic anastomosis without a detectable change in total collagen content.
AB - PURPOSE: Intracolonic infusions of short chain fatty acids promote healing of colonic anastomoses. Because the intravenous route may have wider clinical application, we studied the effect of intravenous n-butyrate on the mechanical strength of colonic anastomoses in the rat. METHODS: After placement of an indwelling intravenous catheter, the descending colon was transected and an anastomosis was performed. Rats were then randomized to receive total parenteral nutrition (TPN group; n = 15) or total parenteral nutrition plus 130 mM/l of n-butyrate (TPN+BUT group; n = 13). On the fifth postoperative day, bursting pressure and bowel wall tension of the anastomoses were measured in situ. Anastomotic tissues were analyzed for hydroxyproline. RESULTS: The TPN+BUT group had a significantly higher bursting pressure (107.5 ± 30.3 vs. 83 ± 41.0 mmHg; P = 0.04) and bowel wall tension (20.7 ± 7.6 vs. 14.1 ± 9.9 Newton; P = 0.03). Tissue hydroxyproline was not different between the two groups (TPN, 45.8 ± 9.2, and TPN+BUT, 47.9 ± 2.9 μg/mg tissue nitrogen). CONCLUSIONS: We conclude that intravenous butyrate improves mechanical strength of a colonic anastomosis without a detectable change in total collagen content.
UR - http://www.scopus.com/inward/record.url?scp=0031028050&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031028050&partnerID=8YFLogxK
U2 - 10.1007/BF02055684
DO - 10.1007/BF02055684
M3 - Article
C2 - 9102264
AN - SCOPUS:0031028050
VL - 40
SP - 67
EP - 70
JO - Diseases of the Colon and Rectum
JF - Diseases of the Colon and Rectum
SN - 0012-3706
IS - 1
ER -