Increased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells

Bauer E. Sumpio, Mark Widmann, Joseph Ricotta, Mark A. Awolesi, Makoto Watase

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Very little is known about the effects of pressure within the vascular system on EC phenotype. To study this, bovine aortic EC were seeded on rat type I collagen plates (2,000/cm2) and allowed to attach for 24 hours. The cells were exposed to either atmospheric, 40, 80, or 120 mm Hg pressure by placing them in a plexiglass pressure chamber loaded with 5% CO2/air and maintained at 37°C inside an incubator. Chamber pressure was continuously monitored with an amplified voltage transducer connected to a digital monitor. EC were maintained in DMEM supplemented with 10% calf serum and substrates for up to 9 days. The results indicate that EC proliferation is influenced by their ambient pressure. EC subjected in vitro to pressures comparable to mean systemic blood pressures had a significant increase in cell number compared to EC exposed to atmospheric pressures. EC were elongated and appeared to align randomly. We hypothesize that the systemic pressure which the endothelium is exposed to in vivo may have a significant regulatory influence on the ability of the endothelium to proliferate which may affect the endothelial cell response to injury. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)133-139
Number of pages7
JournalJournal of Cellular Physiology
Volume158
Issue number1
DOIs
StatePublished - Jan 1 1994

Fingerprint

Endothelial cells
Blood pressure
Polymethyl Methacrylate
Collagen Type I
Atmospheric pressure
Rats
Transducers
Cultured Cells
Endothelial Cells
Pressure
Electric potential
Substrates
Air
Endothelium
Incubators
Atmospheric Pressure
Blood Vessels
Cell Count
Blood Pressure
Phenotype

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Sumpio, Bauer E. ; Widmann, Mark ; Ricotta, Joseph ; Awolesi, Mark A. ; Watase, Makoto. / Increased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells. In: Journal of Cellular Physiology. 1994 ; Vol. 158, No. 1. pp. 133-139.
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Increased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells. / Sumpio, Bauer E.; Widmann, Mark; Ricotta, Joseph; Awolesi, Mark A.; Watase, Makoto.

In: Journal of Cellular Physiology, Vol. 158, No. 1, 01.01.1994, p. 133-139.

Research output: Contribution to journalArticle

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