In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis

Danny O. Jacobs, John Maris, Robert Fried, R. Gregg Settle, Rolando R. Rolandelli, Mark J. Koruda, Briton Chance, John L. Rombeau

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

High-energy phosphate metabolism in skeletal muscle is altered during sepsis, although the chronology of events is uncertain. Phosphorus 31 magnetic resonance spectroscopy was used to measure changes in muscle energy stores of the left hind limb musculature of adult male rats during sepsis. Following control scans, cecal ligation and puncture were performed and scanning was repeated 6, 24, and 48 hours after surgery. The ratios of phosphocreatine (PCr) to inorganic phosphate (Pi), a measure of energy stores, and adenosine triphosphate (ATP) to Pi ratio, a measure of the energy available for immediate use, were determined from peak heights. Intracellular pH was calculated using the distance between Pi and PCr peaks. In surviving animals, a 40% decrease in PCr/Pi ratio (±SEM) was observed by 24 hours (22.3±3.0 at time 0 vs 13.3±2.8 at 24 hours), whereas energy availability (β-ATP/Pi) was statistically unchanged (18.2 ±2.2 at time 0 vs 15.2± 1.2 at 48 hours). Intracellular pH did not change. Both PCr/Pi and ATP/Pi ratios were inversely correlated with time. In this model of documented peritonitis, skeletal muscle energy metabolism is rapidly altered following severe infection, and these changes can be detected using 31P magnetic resonance spectroscopy.

Original languageEnglish (US)
Pages (from-to)1425-1428
Number of pages4
JournalArchives of Surgery
Volume123
Issue number11
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Phosphocreatine
Phosphorus
Sepsis
Skeletal Muscle
Magnetic Resonance Spectroscopy
Extremities
Adenosine Triphosphate
Energy Metabolism
Phosphates
Chronology
Peritonitis
Punctures
Ligation
Muscles
Infection

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Jacobs, Danny O. ; Maris, John ; Fried, Robert ; Settle, R. Gregg ; Rolandelli, Rolando R. ; Koruda, Mark J. ; Chance, Briton ; Rombeau, John L. / In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis. In: Archives of Surgery. 1988 ; Vol. 123, No. 11. pp. 1425-1428.
@article{d133ed4d2e064c0b8875ff9f6e93ef5c,
title = "In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis",
abstract = "High-energy phosphate metabolism in skeletal muscle is altered during sepsis, although the chronology of events is uncertain. Phosphorus 31 magnetic resonance spectroscopy was used to measure changes in muscle energy stores of the left hind limb musculature of adult male rats during sepsis. Following control scans, cecal ligation and puncture were performed and scanning was repeated 6, 24, and 48 hours after surgery. The ratios of phosphocreatine (PCr) to inorganic phosphate (Pi), a measure of energy stores, and adenosine triphosphate (ATP) to Pi ratio, a measure of the energy available for immediate use, were determined from peak heights. Intracellular pH was calculated using the distance between Pi and PCr peaks. In surviving animals, a 40{\%} decrease in PCr/Pi ratio (±SEM) was observed by 24 hours (22.3±3.0 at time 0 vs 13.3±2.8 at 24 hours), whereas energy availability (β-ATP/Pi) was statistically unchanged (18.2 ±2.2 at time 0 vs 15.2± 1.2 at 48 hours). Intracellular pH did not change. Both PCr/Pi and ATP/Pi ratios were inversely correlated with time. In this model of documented peritonitis, skeletal muscle energy metabolism is rapidly altered following severe infection, and these changes can be detected using 31P magnetic resonance spectroscopy.",
author = "Jacobs, {Danny O.} and John Maris and Robert Fried and Settle, {R. Gregg} and Rolandelli, {Rolando R.} and Koruda, {Mark J.} and Briton Chance and Rombeau, {John L.}",
year = "1988",
month = "1",
day = "1",
doi = "10.1001/archsurg.1988.01400350139022",
language = "English (US)",
volume = "123",
pages = "1425--1428",
journal = "JAMA Surgery",
issn = "2168-6254",
publisher = "American Medical Association",
number = "11",

}

Jacobs, DO, Maris, J, Fried, R, Settle, RG, Rolandelli, RR, Koruda, MJ, Chance, B & Rombeau, JL 1988, 'In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis', Archives of Surgery, vol. 123, no. 11, pp. 1425-1428. https://doi.org/10.1001/archsurg.1988.01400350139022

In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis. / Jacobs, Danny O.; Maris, John; Fried, Robert; Settle, R. Gregg; Rolandelli, Rolando R.; Koruda, Mark J.; Chance, Briton; Rombeau, John L.

In: Archives of Surgery, Vol. 123, No. 11, 01.01.1988, p. 1425-1428.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis

AU - Jacobs, Danny O.

AU - Maris, John

AU - Fried, Robert

AU - Settle, R. Gregg

AU - Rolandelli, Rolando R.

AU - Koruda, Mark J.

AU - Chance, Briton

AU - Rombeau, John L.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - High-energy phosphate metabolism in skeletal muscle is altered during sepsis, although the chronology of events is uncertain. Phosphorus 31 magnetic resonance spectroscopy was used to measure changes in muscle energy stores of the left hind limb musculature of adult male rats during sepsis. Following control scans, cecal ligation and puncture were performed and scanning was repeated 6, 24, and 48 hours after surgery. The ratios of phosphocreatine (PCr) to inorganic phosphate (Pi), a measure of energy stores, and adenosine triphosphate (ATP) to Pi ratio, a measure of the energy available for immediate use, were determined from peak heights. Intracellular pH was calculated using the distance between Pi and PCr peaks. In surviving animals, a 40% decrease in PCr/Pi ratio (±SEM) was observed by 24 hours (22.3±3.0 at time 0 vs 13.3±2.8 at 24 hours), whereas energy availability (β-ATP/Pi) was statistically unchanged (18.2 ±2.2 at time 0 vs 15.2± 1.2 at 48 hours). Intracellular pH did not change. Both PCr/Pi and ATP/Pi ratios were inversely correlated with time. In this model of documented peritonitis, skeletal muscle energy metabolism is rapidly altered following severe infection, and these changes can be detected using 31P magnetic resonance spectroscopy.

AB - High-energy phosphate metabolism in skeletal muscle is altered during sepsis, although the chronology of events is uncertain. Phosphorus 31 magnetic resonance spectroscopy was used to measure changes in muscle energy stores of the left hind limb musculature of adult male rats during sepsis. Following control scans, cecal ligation and puncture were performed and scanning was repeated 6, 24, and 48 hours after surgery. The ratios of phosphocreatine (PCr) to inorganic phosphate (Pi), a measure of energy stores, and adenosine triphosphate (ATP) to Pi ratio, a measure of the energy available for immediate use, were determined from peak heights. Intracellular pH was calculated using the distance between Pi and PCr peaks. In surviving animals, a 40% decrease in PCr/Pi ratio (±SEM) was observed by 24 hours (22.3±3.0 at time 0 vs 13.3±2.8 at 24 hours), whereas energy availability (β-ATP/Pi) was statistically unchanged (18.2 ±2.2 at time 0 vs 15.2± 1.2 at 48 hours). Intracellular pH did not change. Both PCr/Pi and ATP/Pi ratios were inversely correlated with time. In this model of documented peritonitis, skeletal muscle energy metabolism is rapidly altered following severe infection, and these changes can be detected using 31P magnetic resonance spectroscopy.

UR - http://www.scopus.com/inward/record.url?scp=0023790497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023790497&partnerID=8YFLogxK

U2 - 10.1001/archsurg.1988.01400350139022

DO - 10.1001/archsurg.1988.01400350139022

M3 - Article

C2 - 3178491

AN - SCOPUS:0023790497

VL - 123

SP - 1425

EP - 1428

JO - JAMA Surgery

JF - JAMA Surgery

SN - 2168-6254

IS - 11

ER -