Hyperthermia in combination with oxidative stress induces autophagic cell death in HT-29 colon cancer cells

Fei Chen, Chia Chi Wang, Eugene Kim, Lawrence E. Harrison

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the mechanism of ROS-induced hyperthermic cell death in a colon cancer cell line. HT-29 colon cancer cells were exposed to heat (43 °C) in the presence of tert-butyl hydroperoxide (t-BOOH). t-BOOH combined with hyperthermia significantly decreased cell viability as compared with t-BOOH or hyperthermia alone. This decrease in cell numbers was associated with retardation in the S phase transit and not through apoptosis. Cell death was noted to be accompanied by specific features characteristic of autophagy: the presence of cytoplasmic autophagic vacuoles; autophagosome membrane association of microtubule-associated protein light chain 3; accumulation of acidic vesicular organelles; and increased incorporation of MDC in the autophagosome. Thermal sensitization through modulation of cellular ROS may represent a novel approach to increase the efficacy of hyperthermia as an anticancer modality.

Original languageEnglish (US)
Pages (from-to)715-723
Number of pages9
JournalCell Biology International
Volume32
Issue number7
DOIs
StatePublished - Jul 1 2008
Externally publishedYes

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Autophagy
Colonic Neoplasms
Oxidative Stress
Fever
Cell Death
Hot Temperature
tert-Butylhydroperoxide
Microtubule-Associated Proteins
Vacuoles
S Phase
Organelles
Cell Survival
Cell Count
Apoptosis
Light
Cell Line
Membranes
Autophagosomes

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

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Hyperthermia in combination with oxidative stress induces autophagic cell death in HT-29 colon cancer cells. / Chen, Fei; Wang, Chia Chi; Kim, Eugene; Harrison, Lawrence E.

In: Cell Biology International, Vol. 32, No. 7, 01.07.2008, p. 715-723.

Research output: Contribution to journalArticle

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