Epigenetic alteration of p16INK4a gene in dedifferentiation of liposarcoma

Mai He, Seena Aisner, Joseph Benevenia, Francis Patterson, Lawrence E. Harrison, Meera Hameed

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The atypical lipomatous tumor (ALT)/well-differentiated liposarcoma (WDLPS) is a locally aggressive subtype of liposarcoma unless dedifferentiation occurs. The mechanism driving this progression is not clear. Loss of p16 is believed to be an early and critical event in tumor progression. Gene silencing by methylation of p16INK4a gene promoter has been reported in several soft tissue sarcomas. The aim of this study is to study the role of p16INK4a gene promoter methylation and p16 expression in tumor progression (dedifferentiation) and recurrence of ALT/WDLPS. Four cases of dedifferentiated liposarcomas (DDLPS) and three cases of recurrent well-differentiated liposarcomas (WDLPS) were collected, and methylation status of p16INK4a gene promoter was analyzed using methylation-specific PCR (MSP) on DNA extracted from paraffin blocks. p16 expression was examined by immunohistochemistry on the same blocks. Methylation of p16INK4a gene promoter was seen in the dedifferentiated (DD) components only, in two out of four (2/4, 50%) DDLPS. The other two DDLPS and three recurrent WDLPS were not methylated. Both WD and DD components in all four DDLPS cases showed strong nuclear p16 expression. All three recurrent WDLPS showed positive p16 expression with similar intensity between primary and recurrent tumors. Even though linear correlation between p16 promoter hypermethylation and p16 protein expression was not present, there appears to be a role for p16INK4a gene promoter hypermethylation in DDLPS and not in recurrent WDLPS.

Original languageEnglish (US)
Pages (from-to)386-394
Number of pages9
JournalPathology Research and Practice
Volume205
Issue number6
DOIs
StatePublished - Jun 15 2009
Externally publishedYes

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p16 Genes
Liposarcoma
Epigenomics
Methylation
Neoplasms
Gene Silencing
Sarcoma
Paraffin

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

He, Mai ; Aisner, Seena ; Benevenia, Joseph ; Patterson, Francis ; Harrison, Lawrence E. ; Hameed, Meera. / Epigenetic alteration of p16INK4a gene in dedifferentiation of liposarcoma. In: Pathology Research and Practice. 2009 ; Vol. 205, No. 6. pp. 386-394.
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Epigenetic alteration of p16INK4a gene in dedifferentiation of liposarcoma. / He, Mai; Aisner, Seena; Benevenia, Joseph; Patterson, Francis; Harrison, Lawrence E.; Hameed, Meera.

In: Pathology Research and Practice, Vol. 205, No. 6, 15.06.2009, p. 386-394.

Research output: Contribution to journalArticle

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T1 - Epigenetic alteration of p16INK4a gene in dedifferentiation of liposarcoma

AU - He, Mai

AU - Aisner, Seena

AU - Benevenia, Joseph

AU - Patterson, Francis

AU - Harrison, Lawrence E.

AU - Hameed, Meera

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AB - The atypical lipomatous tumor (ALT)/well-differentiated liposarcoma (WDLPS) is a locally aggressive subtype of liposarcoma unless dedifferentiation occurs. The mechanism driving this progression is not clear. Loss of p16 is believed to be an early and critical event in tumor progression. Gene silencing by methylation of p16INK4a gene promoter has been reported in several soft tissue sarcomas. The aim of this study is to study the role of p16INK4a gene promoter methylation and p16 expression in tumor progression (dedifferentiation) and recurrence of ALT/WDLPS. Four cases of dedifferentiated liposarcomas (DDLPS) and three cases of recurrent well-differentiated liposarcomas (WDLPS) were collected, and methylation status of p16INK4a gene promoter was analyzed using methylation-specific PCR (MSP) on DNA extracted from paraffin blocks. p16 expression was examined by immunohistochemistry on the same blocks. Methylation of p16INK4a gene promoter was seen in the dedifferentiated (DD) components only, in two out of four (2/4, 50%) DDLPS. The other two DDLPS and three recurrent WDLPS were not methylated. Both WD and DD components in all four DDLPS cases showed strong nuclear p16 expression. All three recurrent WDLPS showed positive p16 expression with similar intensity between primary and recurrent tumors. Even though linear correlation between p16 promoter hypermethylation and p16 protein expression was not present, there appears to be a role for p16INK4a gene promoter hypermethylation in DDLPS and not in recurrent WDLPS.

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