Effect of cyclic stretch on endothelial cells from different vascular beds

T. Iba, S. Maitz, T. Furbert, O. Rosales, M. D. Widmann, B. Spillane, T. Shin, T. Sonoda, B. E. Sumpio

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Endothelial cells (EC) mediate many of the organ responses to shock. Much of our knowledge of EC are obtained from cell culture studies. However, compared to the dynamic milieu in vivo, the stationary environment for large- vessel EC may be artificial and inappropriate. In this study, the morphology, growth rate, and production of prostacyclin (PGI2) by EC obtained from different vascular beds under stationary and dynamic conditions were examined. EC were harvested from the thoracic aorta (Ao), pulmonary artery (PA), and vena cava (VC) of the same calves and exposed to 0.5 sec 24% deformation alternating with 0.5 sec relaxation (i.e., 60 cycles/min). Our results show that in response to the cyclic regimen, VCEC were elongated perpendicular to the force vector and their actin filaments aligned in the same direction, while AoEC and PAEC did not exhibit any morphological changes. The growth rate of AoEC (but not PAEC or VCEC) was significantly enhanced when stimulated by cyclic stretch. In addition, AoEC demonstrated an increased PGI2 synthetic activity with cyclic stretch, while PAEC and VCEC were unaltered. We conclude that the maintenance of EC phenotype and function is dependent on the hemodynamic milieu in vivo and may be influenced by the vascular origin of the cultured EC.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalCirculatory Shock
Volume35
Issue number4
StatePublished - Dec 1 1991
Externally publishedYes

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Endothelial Cells
Epoprostenol
Blood Vessels
Venae Cavae
Growth
Thoracic Aorta
Actin Cytoskeleton
Pulmonary Artery
Cultured Cells
Shock
Cell Culture Techniques
Hemodynamics
Maintenance
Phenotype

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Iba, T., Maitz, S., Furbert, T., Rosales, O., Widmann, M. D., Spillane, B., ... Sumpio, B. E. (1991). Effect of cyclic stretch on endothelial cells from different vascular beds. Circulatory Shock, 35(4), 193-198.
Iba, T. ; Maitz, S. ; Furbert, T. ; Rosales, O. ; Widmann, M. D. ; Spillane, B. ; Shin, T. ; Sonoda, T. ; Sumpio, B. E. / Effect of cyclic stretch on endothelial cells from different vascular beds. In: Circulatory Shock. 1991 ; Vol. 35, No. 4. pp. 193-198.
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Iba, T, Maitz, S, Furbert, T, Rosales, O, Widmann, MD, Spillane, B, Shin, T, Sonoda, T & Sumpio, BE 1991, 'Effect of cyclic stretch on endothelial cells from different vascular beds', Circulatory Shock, vol. 35, no. 4, pp. 193-198.

Effect of cyclic stretch on endothelial cells from different vascular beds. / Iba, T.; Maitz, S.; Furbert, T.; Rosales, O.; Widmann, M. D.; Spillane, B.; Shin, T.; Sonoda, T.; Sumpio, B. E.

In: Circulatory Shock, Vol. 35, No. 4, 01.12.1991, p. 193-198.

Research output: Contribution to journalArticle

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T1 - Effect of cyclic stretch on endothelial cells from different vascular beds

AU - Iba, T.

AU - Maitz, S.

AU - Furbert, T.

AU - Rosales, O.

AU - Widmann, M. D.

AU - Spillane, B.

AU - Shin, T.

AU - Sonoda, T.

AU - Sumpio, B. E.

PY - 1991/12/1

Y1 - 1991/12/1

N2 - Endothelial cells (EC) mediate many of the organ responses to shock. Much of our knowledge of EC are obtained from cell culture studies. However, compared to the dynamic milieu in vivo, the stationary environment for large- vessel EC may be artificial and inappropriate. In this study, the morphology, growth rate, and production of prostacyclin (PGI2) by EC obtained from different vascular beds under stationary and dynamic conditions were examined. EC were harvested from the thoracic aorta (Ao), pulmonary artery (PA), and vena cava (VC) of the same calves and exposed to 0.5 sec 24% deformation alternating with 0.5 sec relaxation (i.e., 60 cycles/min). Our results show that in response to the cyclic regimen, VCEC were elongated perpendicular to the force vector and their actin filaments aligned in the same direction, while AoEC and PAEC did not exhibit any morphological changes. The growth rate of AoEC (but not PAEC or VCEC) was significantly enhanced when stimulated by cyclic stretch. In addition, AoEC demonstrated an increased PGI2 synthetic activity with cyclic stretch, while PAEC and VCEC were unaltered. We conclude that the maintenance of EC phenotype and function is dependent on the hemodynamic milieu in vivo and may be influenced by the vascular origin of the cultured EC.

AB - Endothelial cells (EC) mediate many of the organ responses to shock. Much of our knowledge of EC are obtained from cell culture studies. However, compared to the dynamic milieu in vivo, the stationary environment for large- vessel EC may be artificial and inappropriate. In this study, the morphology, growth rate, and production of prostacyclin (PGI2) by EC obtained from different vascular beds under stationary and dynamic conditions were examined. EC were harvested from the thoracic aorta (Ao), pulmonary artery (PA), and vena cava (VC) of the same calves and exposed to 0.5 sec 24% deformation alternating with 0.5 sec relaxation (i.e., 60 cycles/min). Our results show that in response to the cyclic regimen, VCEC were elongated perpendicular to the force vector and their actin filaments aligned in the same direction, while AoEC and PAEC did not exhibit any morphological changes. The growth rate of AoEC (but not PAEC or VCEC) was significantly enhanced when stimulated by cyclic stretch. In addition, AoEC demonstrated an increased PGI2 synthetic activity with cyclic stretch, while PAEC and VCEC were unaltered. We conclude that the maintenance of EC phenotype and function is dependent on the hemodynamic milieu in vivo and may be influenced by the vascular origin of the cultured EC.

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Iba T, Maitz S, Furbert T, Rosales O, Widmann MD, Spillane B et al. Effect of cyclic stretch on endothelial cells from different vascular beds. Circulatory Shock. 1991 Dec 1;35(4):193-198.