Ecto-5′-nucleotidase (CD73) decreases mortality and organ injury in sepsis

György Haskó, Balázs Csóka, Balázs Koscsó, Rachna Chandra, Pál Pacher, Linda F. Thompson, Edwin A. Deitch, Zoltán Spolarics, László Virág, Pál Gergely, Rolando H. Rolandelli, Zoltán H. Németh

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The extracellular concentrations of adenosine are increased during sepsis, and adenosine receptors regulate the host's response to sepsis. In this study, we investigated the role of the adenosine-generating ectoenzyme, ecto-59-nucleotidase (CD73), in regulating immune and organ function during sepsis. Polymicrobial sepsis was induced by subjecting CD73 knockout (KO) and wild type (WT) mice to cecal ligation and puncture. CD73 KO mice showed increased mortality in comparison with WT mice, which was associated with increased bacterial counts and elevated inflammatory cytokine and chemokine concentrations in the blood and peritoneum. CD73 deficiency promoted lung injury, as indicated by increased myeloperoxidase activity and neutrophil infiltration, and elevated pulmonary cytokine levels. CD73 KO mice had increased apoptosis in the thymus, as evidenced by increased cleavage of caspase-3 and poly(ADP-ribose) polymerase and increased activation of NF-κB. Septic CD73 KO mice had higher blood urea nitrogen levels and increased cytokine levels in the kidney, indicating increased renal dysfunction. The increased kidney injury of CD73 KO mice was associated with augmented activation of p38 MAPK and decreased phosphorylation of Akt. Pharmacological inactivation of CD73 in WT mice using α, β-methylene ADP augmented cytokine levels in the blood and peritoneal lavage fluid. These findings suggest that CD73-derived adenosine may be beneficial in sepsis.

Original languageEnglish (US)
Pages (from-to)4256-4267
Number of pages12
JournalJournal of Immunology
Volume187
Issue number8
DOIs
StatePublished - Oct 15 2011
Externally publishedYes

Fingerprint

5'-Nucleotidase
Knockout Mice
Sepsis
Mortality
Adenosine
Cytokines
Wounds and Injuries
Kidney
Peritoneal Lavage
Purinergic P1 Receptors
Poly(ADP-ribose) Polymerases
Neutrophil Infiltration
Bacterial Load
Ascitic Fluid
Peritoneum
Blood Urea Nitrogen
Lung Injury
p38 Mitogen-Activated Protein Kinases
Punctures
Chemokines

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Haskó, G., Csóka, B., Koscsó, B., Chandra, R., Pacher, P., Thompson, L. F., ... Németh, Z. H. (2011). Ecto-5′-nucleotidase (CD73) decreases mortality and organ injury in sepsis. Journal of Immunology, 187(8), 4256-4267. https://doi.org/10.4049/jimmunol.1003379
Haskó, György ; Csóka, Balázs ; Koscsó, Balázs ; Chandra, Rachna ; Pacher, Pál ; Thompson, Linda F. ; Deitch, Edwin A. ; Spolarics, Zoltán ; Virág, László ; Gergely, Pál ; Rolandelli, Rolando H. ; Németh, Zoltán H. / Ecto-5′-nucleotidase (CD73) decreases mortality and organ injury in sepsis. In: Journal of Immunology. 2011 ; Vol. 187, No. 8. pp. 4256-4267.
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Haskó, G, Csóka, B, Koscsó, B, Chandra, R, Pacher, P, Thompson, LF, Deitch, EA, Spolarics, Z, Virág, L, Gergely, P, Rolandelli, RH & Németh, ZH 2011, 'Ecto-5′-nucleotidase (CD73) decreases mortality and organ injury in sepsis', Journal of Immunology, vol. 187, no. 8, pp. 4256-4267. https://doi.org/10.4049/jimmunol.1003379

Ecto-5′-nucleotidase (CD73) decreases mortality and organ injury in sepsis. / Haskó, György; Csóka, Balázs; Koscsó, Balázs; Chandra, Rachna; Pacher, Pál; Thompson, Linda F.; Deitch, Edwin A.; Spolarics, Zoltán; Virág, László; Gergely, Pál; Rolandelli, Rolando H.; Németh, Zoltán H.

In: Journal of Immunology, Vol. 187, No. 8, 15.10.2011, p. 4256-4267.

Research output: Contribution to journalArticle

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AU - Haskó, György

AU - Csóka, Balázs

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AU - Pacher, Pál

AU - Thompson, Linda F.

AU - Deitch, Edwin A.

AU - Spolarics, Zoltán

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AU - Rolandelli, Rolando H.

AU - Németh, Zoltán H.

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Haskó G, Csóka B, Koscsó B, Chandra R, Pacher P, Thompson LF et al. Ecto-5′-nucleotidase (CD73) decreases mortality and organ injury in sepsis. Journal of Immunology. 2011 Oct 15;187(8):4256-4267. https://doi.org/10.4049/jimmunol.1003379