Depletion of high energy phosphate compounds in the tumor-bearing state and reversal after tumor resection

S. N. Hochwald, Lawrence Harrison, J. L. Port, D. Blumberg, M. F. Brennan, M. Burt

Research output: Contribution to journalArticle

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Abstract

Background. Cancer cachexia is a syndrome manifested by a variety of metabolic abnormalities that include depletion of host energy stores. We studied liver and skeletal muscle high energy phosphate compounds, inorganic phosphorus (Pi), and the energy charge in tumor-bearing (TB), pair fed non- tumor-bearing (NTB), and tumor-bearing resected (RES) rats. Methods. F344 rats were randomized into TB (n = 13), NTB (n = 13), and RES (n = 5) groups. On day 0, the flanks of the TB and RES animals were injected with 1 x 107 methylcholanthrene (MCA)-induced sarcoma cells. On day 19, TB and NTB rat liver and skeletal muscle were analyzed for adenine nucleotides, phosphocreatine, and Pi, and RES animals underwent tumor resection followed by tissue analysis 10 days later. Results. Although the liver adenylate energy charge was maintained, the level of liver adenosine monophosphate was significantly increased and the liver adenosine diphosphate level was decreased in the TB animals (3.55 ± 0.6, 3.70 ± 0.3 μmoles/gm dry weight, p < 0.05, p = 0.05, respectively) when compared with the NTB animals (3.06 ± 0.4, 4.00 ± 0.5 μmoles/gm dry weight, respectively). Muscle adenosine diphosphate levels were significantly decreased in the TB animals (1.57 ± 0.7 μmoles/gm dry weight) as compared with NTB animals (2.23 ± 0.7 μmoles/gm dry weight, p < 0.05). In addition, muscle adenosine triphosphate, phosphocreatine, and phosphocreatine/adenosine triphosphate ratios were significantly decreased in TB animals (19.94 ± 4.5, 81.51 ± 12.8, and 4.20 ± 0.8 μmoles/gm dry weight, respectively) as compared with NTB animals (24.44 ± 1.9, 116.72 ± 7.5, and 4.81 ± 0.4 μmoles/gm dry weight, respectively, p < 0.05) and RES animals (24.08 ± 3.3, 124.10 ± 12.2, and 5.19 ± 0.5 μmoles/gm dry weight, respectively, p < 0.05). Conclusions. These alterations in high energy phosphate compounds in liver and skeletal muscle indicate that although the energy charge is maintained, energy depletion occurs early in the tumor-bearing state. These charges are tumor specific, not related to anorexia, and revert to non-tumor-bearing levels after tumor resection.

Original languageEnglish (US)
Pages (from-to)534-541
Number of pages8
JournalSurgery
Volume120
Issue number3
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

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Phosphates
Neoplasms
Weights and Measures
Phosphocreatine
Liver
Skeletal Muscle
Adenosine Diphosphate
Adenosine Triphosphate
Phosphorus Compounds
Muscles
Cachexia
Methylcholanthrene
Adenine Nucleotides
Inbred F344 Rats
Anorexia
Adenosine Monophosphate
Sarcoma

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Hochwald, S. N. ; Harrison, Lawrence ; Port, J. L. ; Blumberg, D. ; Brennan, M. F. ; Burt, M. / Depletion of high energy phosphate compounds in the tumor-bearing state and reversal after tumor resection. In: Surgery. 1996 ; Vol. 120, No. 3. pp. 534-541.
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title = "Depletion of high energy phosphate compounds in the tumor-bearing state and reversal after tumor resection",
abstract = "Background. Cancer cachexia is a syndrome manifested by a variety of metabolic abnormalities that include depletion of host energy stores. We studied liver and skeletal muscle high energy phosphate compounds, inorganic phosphorus (Pi), and the energy charge in tumor-bearing (TB), pair fed non- tumor-bearing (NTB), and tumor-bearing resected (RES) rats. Methods. F344 rats were randomized into TB (n = 13), NTB (n = 13), and RES (n = 5) groups. On day 0, the flanks of the TB and RES animals were injected with 1 x 107 methylcholanthrene (MCA)-induced sarcoma cells. On day 19, TB and NTB rat liver and skeletal muscle were analyzed for adenine nucleotides, phosphocreatine, and Pi, and RES animals underwent tumor resection followed by tissue analysis 10 days later. Results. Although the liver adenylate energy charge was maintained, the level of liver adenosine monophosphate was significantly increased and the liver adenosine diphosphate level was decreased in the TB animals (3.55 ± 0.6, 3.70 ± 0.3 μmoles/gm dry weight, p < 0.05, p = 0.05, respectively) when compared with the NTB animals (3.06 ± 0.4, 4.00 ± 0.5 μmoles/gm dry weight, respectively). Muscle adenosine diphosphate levels were significantly decreased in the TB animals (1.57 ± 0.7 μmoles/gm dry weight) as compared with NTB animals (2.23 ± 0.7 μmoles/gm dry weight, p < 0.05). In addition, muscle adenosine triphosphate, phosphocreatine, and phosphocreatine/adenosine triphosphate ratios were significantly decreased in TB animals (19.94 ± 4.5, 81.51 ± 12.8, and 4.20 ± 0.8 μmoles/gm dry weight, respectively) as compared with NTB animals (24.44 ± 1.9, 116.72 ± 7.5, and 4.81 ± 0.4 μmoles/gm dry weight, respectively, p < 0.05) and RES animals (24.08 ± 3.3, 124.10 ± 12.2, and 5.19 ± 0.5 μmoles/gm dry weight, respectively, p < 0.05). Conclusions. These alterations in high energy phosphate compounds in liver and skeletal muscle indicate that although the energy charge is maintained, energy depletion occurs early in the tumor-bearing state. These charges are tumor specific, not related to anorexia, and revert to non-tumor-bearing levels after tumor resection.",
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Depletion of high energy phosphate compounds in the tumor-bearing state and reversal after tumor resection. / Hochwald, S. N.; Harrison, Lawrence; Port, J. L.; Blumberg, D.; Brennan, M. F.; Burt, M.

In: Surgery, Vol. 120, No. 3, 01.01.1996, p. 534-541.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Depletion of high energy phosphate compounds in the tumor-bearing state and reversal after tumor resection

AU - Hochwald, S. N.

AU - Harrison, Lawrence

AU - Port, J. L.

AU - Blumberg, D.

AU - Brennan, M. F.

AU - Burt, M.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Background. Cancer cachexia is a syndrome manifested by a variety of metabolic abnormalities that include depletion of host energy stores. We studied liver and skeletal muscle high energy phosphate compounds, inorganic phosphorus (Pi), and the energy charge in tumor-bearing (TB), pair fed non- tumor-bearing (NTB), and tumor-bearing resected (RES) rats. Methods. F344 rats were randomized into TB (n = 13), NTB (n = 13), and RES (n = 5) groups. On day 0, the flanks of the TB and RES animals were injected with 1 x 107 methylcholanthrene (MCA)-induced sarcoma cells. On day 19, TB and NTB rat liver and skeletal muscle were analyzed for adenine nucleotides, phosphocreatine, and Pi, and RES animals underwent tumor resection followed by tissue analysis 10 days later. Results. Although the liver adenylate energy charge was maintained, the level of liver adenosine monophosphate was significantly increased and the liver adenosine diphosphate level was decreased in the TB animals (3.55 ± 0.6, 3.70 ± 0.3 μmoles/gm dry weight, p < 0.05, p = 0.05, respectively) when compared with the NTB animals (3.06 ± 0.4, 4.00 ± 0.5 μmoles/gm dry weight, respectively). Muscle adenosine diphosphate levels were significantly decreased in the TB animals (1.57 ± 0.7 μmoles/gm dry weight) as compared with NTB animals (2.23 ± 0.7 μmoles/gm dry weight, p < 0.05). In addition, muscle adenosine triphosphate, phosphocreatine, and phosphocreatine/adenosine triphosphate ratios were significantly decreased in TB animals (19.94 ± 4.5, 81.51 ± 12.8, and 4.20 ± 0.8 μmoles/gm dry weight, respectively) as compared with NTB animals (24.44 ± 1.9, 116.72 ± 7.5, and 4.81 ± 0.4 μmoles/gm dry weight, respectively, p < 0.05) and RES animals (24.08 ± 3.3, 124.10 ± 12.2, and 5.19 ± 0.5 μmoles/gm dry weight, respectively, p < 0.05). Conclusions. These alterations in high energy phosphate compounds in liver and skeletal muscle indicate that although the energy charge is maintained, energy depletion occurs early in the tumor-bearing state. These charges are tumor specific, not related to anorexia, and revert to non-tumor-bearing levels after tumor resection.

AB - Background. Cancer cachexia is a syndrome manifested by a variety of metabolic abnormalities that include depletion of host energy stores. We studied liver and skeletal muscle high energy phosphate compounds, inorganic phosphorus (Pi), and the energy charge in tumor-bearing (TB), pair fed non- tumor-bearing (NTB), and tumor-bearing resected (RES) rats. Methods. F344 rats were randomized into TB (n = 13), NTB (n = 13), and RES (n = 5) groups. On day 0, the flanks of the TB and RES animals were injected with 1 x 107 methylcholanthrene (MCA)-induced sarcoma cells. On day 19, TB and NTB rat liver and skeletal muscle were analyzed for adenine nucleotides, phosphocreatine, and Pi, and RES animals underwent tumor resection followed by tissue analysis 10 days later. Results. Although the liver adenylate energy charge was maintained, the level of liver adenosine monophosphate was significantly increased and the liver adenosine diphosphate level was decreased in the TB animals (3.55 ± 0.6, 3.70 ± 0.3 μmoles/gm dry weight, p < 0.05, p = 0.05, respectively) when compared with the NTB animals (3.06 ± 0.4, 4.00 ± 0.5 μmoles/gm dry weight, respectively). Muscle adenosine diphosphate levels were significantly decreased in the TB animals (1.57 ± 0.7 μmoles/gm dry weight) as compared with NTB animals (2.23 ± 0.7 μmoles/gm dry weight, p < 0.05). In addition, muscle adenosine triphosphate, phosphocreatine, and phosphocreatine/adenosine triphosphate ratios were significantly decreased in TB animals (19.94 ± 4.5, 81.51 ± 12.8, and 4.20 ± 0.8 μmoles/gm dry weight, respectively) as compared with NTB animals (24.44 ± 1.9, 116.72 ± 7.5, and 4.81 ± 0.4 μmoles/gm dry weight, respectively, p < 0.05) and RES animals (24.08 ± 3.3, 124.10 ± 12.2, and 5.19 ± 0.5 μmoles/gm dry weight, respectively, p < 0.05). Conclusions. These alterations in high energy phosphate compounds in liver and skeletal muscle indicate that although the energy charge is maintained, energy depletion occurs early in the tumor-bearing state. These charges are tumor specific, not related to anorexia, and revert to non-tumor-bearing levels after tumor resection.

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