Cyclic strain increases endothelial nitric oxide synthase activity

M. A. Awolesi, Mark Widmann, W. C. Sessa, B. E. Sumpio, C. Brophy, A. H. Harken, G. Whitman

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Background. Endothelial nitric oxide synthase (eNOS) is an important enzyme that controls the production of a potent vascular smooth muscle relaxing factor, nitric oxide. However, the role of hemodynamic forces (blood pressure, cyclic strain, and shear stress) on the regulation of eNOS has not been fully elucidated. Recently, we showed that cyclic strain increases eNOS gene and protein in cultured bovine aortic endothelial cells (EC). Because an increase in gene transcription and protein synthesis may not necessarily translate into an increase in functional activity, the aim of this study was to determine the effects of cyclic strain on eNOS activity. Methods. EC were seeded onto plates with flexible bottoms that can be deformed by vacuum and were then exposed to 60 cycles/minute of either 24% maximum strain (-20 kPa vacuum) or 10% maximum strain (-5 kPa vacuum) for 24 hours. eNOS activity was assessed, and nitric oxide production was determined (as nitrite) by the Greiss reaction. Results. Twenty-four percent strain, at 60 cycles/min, but not 10% strain significantly increases eNOS activity compared with stationary controls. Both strain regimens increased nitric oxide (as nitrite) in culture media compared with stationary controls, although nitrite in media of EC exposed to high strain were significantly increased compared with the lower strain. Conclusions. Cyclic strain increases eNOS activity in cultured bovine aortic EC. These results may indicate the importance of hemodynamic forces in the regulation of eNOS in vivo.

Original languageEnglish (US)
Pages (from-to)439-445
Number of pages7
JournalSurgery
Volume116
Issue number2
StatePublished - Jan 1 1994
Externally publishedYes

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Nitric Oxide Synthase Type III
Endothelial Cells
Vacuum
Nitrites
Nitric Oxide
Hemodynamics
Vascular Smooth Muscle
Culture Media
Proteins
Blood Pressure
Enzymes

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Awolesi, M. A., Widmann, M., Sessa, W. C., Sumpio, B. E., Brophy, C., Harken, A. H., & Whitman, G. (1994). Cyclic strain increases endothelial nitric oxide synthase activity. Surgery, 116(2), 439-445.
Awolesi, M. A. ; Widmann, Mark ; Sessa, W. C. ; Sumpio, B. E. ; Brophy, C. ; Harken, A. H. ; Whitman, G. / Cyclic strain increases endothelial nitric oxide synthase activity. In: Surgery. 1994 ; Vol. 116, No. 2. pp. 439-445.
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abstract = "Background. Endothelial nitric oxide synthase (eNOS) is an important enzyme that controls the production of a potent vascular smooth muscle relaxing factor, nitric oxide. However, the role of hemodynamic forces (blood pressure, cyclic strain, and shear stress) on the regulation of eNOS has not been fully elucidated. Recently, we showed that cyclic strain increases eNOS gene and protein in cultured bovine aortic endothelial cells (EC). Because an increase in gene transcription and protein synthesis may not necessarily translate into an increase in functional activity, the aim of this study was to determine the effects of cyclic strain on eNOS activity. Methods. EC were seeded onto plates with flexible bottoms that can be deformed by vacuum and were then exposed to 60 cycles/minute of either 24{\%} maximum strain (-20 kPa vacuum) or 10{\%} maximum strain (-5 kPa vacuum) for 24 hours. eNOS activity was assessed, and nitric oxide production was determined (as nitrite) by the Greiss reaction. Results. Twenty-four percent strain, at 60 cycles/min, but not 10{\%} strain significantly increases eNOS activity compared with stationary controls. Both strain regimens increased nitric oxide (as nitrite) in culture media compared with stationary controls, although nitrite in media of EC exposed to high strain were significantly increased compared with the lower strain. Conclusions. Cyclic strain increases eNOS activity in cultured bovine aortic EC. These results may indicate the importance of hemodynamic forces in the regulation of eNOS in vivo.",
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Awolesi, MA, Widmann, M, Sessa, WC, Sumpio, BE, Brophy, C, Harken, AH & Whitman, G 1994, 'Cyclic strain increases endothelial nitric oxide synthase activity', Surgery, vol. 116, no. 2, pp. 439-445.

Cyclic strain increases endothelial nitric oxide synthase activity. / Awolesi, M. A.; Widmann, Mark; Sessa, W. C.; Sumpio, B. E.; Brophy, C.; Harken, A. H.; Whitman, G.

In: Surgery, Vol. 116, No. 2, 01.01.1994, p. 439-445.

Research output: Contribution to journalArticle

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T1 - Cyclic strain increases endothelial nitric oxide synthase activity

AU - Awolesi, M. A.

AU - Widmann, Mark

AU - Sessa, W. C.

AU - Sumpio, B. E.

AU - Brophy, C.

AU - Harken, A. H.

AU - Whitman, G.

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N2 - Background. Endothelial nitric oxide synthase (eNOS) is an important enzyme that controls the production of a potent vascular smooth muscle relaxing factor, nitric oxide. However, the role of hemodynamic forces (blood pressure, cyclic strain, and shear stress) on the regulation of eNOS has not been fully elucidated. Recently, we showed that cyclic strain increases eNOS gene and protein in cultured bovine aortic endothelial cells (EC). Because an increase in gene transcription and protein synthesis may not necessarily translate into an increase in functional activity, the aim of this study was to determine the effects of cyclic strain on eNOS activity. Methods. EC were seeded onto plates with flexible bottoms that can be deformed by vacuum and were then exposed to 60 cycles/minute of either 24% maximum strain (-20 kPa vacuum) or 10% maximum strain (-5 kPa vacuum) for 24 hours. eNOS activity was assessed, and nitric oxide production was determined (as nitrite) by the Greiss reaction. Results. Twenty-four percent strain, at 60 cycles/min, but not 10% strain significantly increases eNOS activity compared with stationary controls. Both strain regimens increased nitric oxide (as nitrite) in culture media compared with stationary controls, although nitrite in media of EC exposed to high strain were significantly increased compared with the lower strain. Conclusions. Cyclic strain increases eNOS activity in cultured bovine aortic EC. These results may indicate the importance of hemodynamic forces in the regulation of eNOS in vivo.

AB - Background. Endothelial nitric oxide synthase (eNOS) is an important enzyme that controls the production of a potent vascular smooth muscle relaxing factor, nitric oxide. However, the role of hemodynamic forces (blood pressure, cyclic strain, and shear stress) on the regulation of eNOS has not been fully elucidated. Recently, we showed that cyclic strain increases eNOS gene and protein in cultured bovine aortic endothelial cells (EC). Because an increase in gene transcription and protein synthesis may not necessarily translate into an increase in functional activity, the aim of this study was to determine the effects of cyclic strain on eNOS activity. Methods. EC were seeded onto plates with flexible bottoms that can be deformed by vacuum and were then exposed to 60 cycles/minute of either 24% maximum strain (-20 kPa vacuum) or 10% maximum strain (-5 kPa vacuum) for 24 hours. eNOS activity was assessed, and nitric oxide production was determined (as nitrite) by the Greiss reaction. Results. Twenty-four percent strain, at 60 cycles/min, but not 10% strain significantly increases eNOS activity compared with stationary controls. Both strain regimens increased nitric oxide (as nitrite) in culture media compared with stationary controls, although nitrite in media of EC exposed to high strain were significantly increased compared with the lower strain. Conclusions. Cyclic strain increases eNOS activity in cultured bovine aortic EC. These results may indicate the importance of hemodynamic forces in the regulation of eNOS in vivo.

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Awolesi MA, Widmann M, Sessa WC, Sumpio BE, Brophy C, Harken AH et al. Cyclic strain increases endothelial nitric oxide synthase activity. Surgery. 1994 Jan 1;116(2):439-445.