Correlation of Biomarker Expression in Colonic Mucosa with Disease Phenotype in Crohn’s Disease and Ulcerative Colitis

Maria E.C. Bruno, Eric W. Rogier, Razvan Arsenescu, Deborah R. Flomenhoft, Cathryn J. Kurkjian, Gavin I. Ellis, Charlotte S. Kaetzel

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Inflammatory bowel diseases (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), are characterized by chronic intestinal inflammation due to immunological, microbial, and environmental factors in genetically predisposed individuals. Advances in the diagnosis, prognosis, and treatment of IBD require the identification of robust biomarkers that can be used for molecular classification of diverse disease presentations. We previously identified five genes, RELA, TNFAIP3 (A20), PIGR, TNF, and IL8, whose mRNA levels in colonic mucosal biopsies could be used in a multivariate analysis to classify patients with CD based on disease behavior and responses to therapy. Aim: We compared expression of these five biomarkers in IBD patients classified as having CD or UC, and in healthy controls. Results: Patients with CD were characterized as having decreased median expression of TNFAIP3, PIGR, and TNF in non-inflamed colonic mucosa as compared to healthy controls. By contrast, UC patients exhibited decreased expression of PIGR and elevated expression of IL8 in colonic mucosa compared to healthy controls. A multivariate analysis combining mRNA levels for all five genes resulted in segregation of individuals based on disease presentation (CD vs. UC) as well as severity, i.e., patients in remission versus those with acute colitis at the time of biopsy. Conclusion: We propose that this approach could be used as a model for molecular classification of IBD patients, which could further be enhanced by the inclusion of additional genes that are identified by functional studies, global gene expression analyses, and genome-wide association studies.

Original languageEnglish (US)
Pages (from-to)2976-2984
Number of pages9
JournalDigestive Diseases and Sciences
Volume60
Issue number10
DOIs
StatePublished - Oct 25 2015
Externally publishedYes

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Ulcerative Colitis
Crohn Disease
Mucous Membrane
Biomarkers
Phenotype
Inflammatory Bowel Diseases
Interleukin-8
Multivariate Analysis
Genes
Biopsy
Messenger RNA
Molecular Models
Genome-Wide Association Study
Colitis
Inflammation
Gene Expression
Therapeutics

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

Cite this

Bruno, Maria E.C. ; Rogier, Eric W. ; Arsenescu, Razvan ; Flomenhoft, Deborah R. ; Kurkjian, Cathryn J. ; Ellis, Gavin I. ; Kaetzel, Charlotte S. / Correlation of Biomarker Expression in Colonic Mucosa with Disease Phenotype in Crohn’s Disease and Ulcerative Colitis. In: Digestive Diseases and Sciences. 2015 ; Vol. 60, No. 10. pp. 2976-2984.
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Correlation of Biomarker Expression in Colonic Mucosa with Disease Phenotype in Crohn’s Disease and Ulcerative Colitis. / Bruno, Maria E.C.; Rogier, Eric W.; Arsenescu, Razvan; Flomenhoft, Deborah R.; Kurkjian, Cathryn J.; Ellis, Gavin I.; Kaetzel, Charlotte S.

In: Digestive Diseases and Sciences, Vol. 60, No. 10, 25.10.2015, p. 2976-2984.

Research output: Contribution to journalArticle

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AU - Rogier, Eric W.

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