Butyrate-induced G2/M block in caco-2 colon cancer cells is associated with decreased p34cdc2 activity

Lawrence Harrison, Qing Mei Wang, George P. Studzinski

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Butyrate, a short-chain fatty acid, has been reported to inhibit proliferation and stimulate differentiation in multiple cancer cell lines. Whereas the effects of butyrate on cellular differentiation are well documented, the relationship between butyrate-induced differentiation and its effect on cell cycle traverse is less well understood. The purpose of this study was to investigate the effects of butyrate on the regulatory proteins of the G2/M traverse in the Caco-2 colon cancer cell model. We demonstrated that the inhibition of proliferation and increased cellular differentiation after treatment of Caco-2 cells with butyrate were associated with a significant G2/M cell cycle block. Although protein levels of the major G2/M regulatory protein, p34cdc2, were unchanged, a decrease in p34cdc2 activity was noted. Despite this decrease in activity, the inhibitory tyrosine phosphorylation of p34cdc2 was decreased, suggesting that other factors are responsible for the decreased kinase activity. The reduced activity of p34cdc2 provides a possible mechanism for the accumulation of Caco-2 cells in the G2/M cell cycle compartment following exposure to butyrate. This cell system provides a new model for studies of G2/M cell cycle perturbations.

Original languageEnglish (US)
Pages (from-to)150-156
Number of pages7
JournalExperimental Biology and Medicine
Volume222
Issue number2
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

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Butyrates
Colonic Neoplasms
Cells
Cell Cycle
Caco-2 Cells
CDC2 Protein Kinase
Phosphorylation
Volatile Fatty Acids
Tyrosine
Proteins
Phosphotransferases
Cell Proliferation
Cell Line
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "Butyrate, a short-chain fatty acid, has been reported to inhibit proliferation and stimulate differentiation in multiple cancer cell lines. Whereas the effects of butyrate on cellular differentiation are well documented, the relationship between butyrate-induced differentiation and its effect on cell cycle traverse is less well understood. The purpose of this study was to investigate the effects of butyrate on the regulatory proteins of the G2/M traverse in the Caco-2 colon cancer cell model. We demonstrated that the inhibition of proliferation and increased cellular differentiation after treatment of Caco-2 cells with butyrate were associated with a significant G2/M cell cycle block. Although protein levels of the major G2/M regulatory protein, p34cdc2, were unchanged, a decrease in p34cdc2 activity was noted. Despite this decrease in activity, the inhibitory tyrosine phosphorylation of p34cdc2 was decreased, suggesting that other factors are responsible for the decreased kinase activity. The reduced activity of p34cdc2 provides a possible mechanism for the accumulation of Caco-2 cells in the G2/M cell cycle compartment following exposure to butyrate. This cell system provides a new model for studies of G2/M cell cycle perturbations.",
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Butyrate-induced G2/M block in caco-2 colon cancer cells is associated with decreased p34cdc2 activity. / Harrison, Lawrence; Wang, Qing Mei; Studzinski, George P.

In: Experimental Biology and Medicine, Vol. 222, No. 2, 01.01.1999, p. 150-156.

Research output: Contribution to journalArticle

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