Assessing burn wound depth using in vitro nuclear magnetic resonance (NMR)

Mark J. Koruda, Andrea Zimbler, R. Gregg Settle, Danny O. Jacobs, Rolando Rolandelli, Gerald L. Wolf, John L. Rombeau

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

There is no accurate noninvasive method for differentiating between partial-thickness and full-thickness cutaneous burn wounds. Full-thickness burns (FTB) result in slower resorption of wound edema than partial thickness burns (PTB). Since proton NMR parameters, particularly the T1 relaxation time, correlate with tissue water content (TWC), the present study determined whether proton NMR could distinguish PTB from FTB. An area of upper dorsum (∼ 15% BSA) of 35 adult rats was immersed in boiling water for either 3 sec (PTB) or 10 sec (FTB). In 10 control rats, the same area was immersed in room temperature water. Rats were sacrificed at either 3 or 48 hr after burn, and skin samples were analyzed to determine spin-lattice (T1) and spin-spin (T2) relaxation times. TWC was then measured gravimetrically by desiccation. Both T1 and T2 times significantly correlated with TWC (T1: r = 0.74, P < 0.0001; T2: r = 0.75; P < 0.0001). Both PTB and FTB resulted in significant elevations of T1, T2, and TWC 3 hr after injury (P > 0.001). At 48 hr postburn the T1, T2, and TWC of the PTB group had decreased to control values (P > 0.05), while all FTB parameters remained significantly elevated as compared to both the control and the 48-hr PTB parameters (P < 0.001). In vitro NMR measurements distinguish PTB from FTB in this rat model within 48 hr. These data provide a basis for investigating in vitro NMR techniques for the noninvasive assessment of burn wound depth.

Original languageEnglish (US)
Pages (from-to)475-481
Number of pages7
JournalJournal of Surgical Research
Volume40
Issue number5
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Burns
Magnetic Resonance Spectroscopy
Wounds and Injuries
Water
Protons
In Vitro Techniques
Desiccation
Skin
Edema
Temperature

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Koruda, M. J., Zimbler, A., Settle, R. G., Jacobs, D. O., Rolandelli, R., Wolf, G. L., & Rombeau, J. L. (1986). Assessing burn wound depth using in vitro nuclear magnetic resonance (NMR). Journal of Surgical Research, 40(5), 475-481. https://doi.org/10.1016/0022-4804(86)90218-0
Koruda, Mark J. ; Zimbler, Andrea ; Settle, R. Gregg ; Jacobs, Danny O. ; Rolandelli, Rolando ; Wolf, Gerald L. ; Rombeau, John L. / Assessing burn wound depth using in vitro nuclear magnetic resonance (NMR). In: Journal of Surgical Research. 1986 ; Vol. 40, No. 5. pp. 475-481.
@article{c0f024e8b52e4564b1db476734bd4e9e,
title = "Assessing burn wound depth using in vitro nuclear magnetic resonance (NMR)",
abstract = "There is no accurate noninvasive method for differentiating between partial-thickness and full-thickness cutaneous burn wounds. Full-thickness burns (FTB) result in slower resorption of wound edema than partial thickness burns (PTB). Since proton NMR parameters, particularly the T1 relaxation time, correlate with tissue water content (TWC), the present study determined whether proton NMR could distinguish PTB from FTB. An area of upper dorsum (∼ 15{\%} BSA) of 35 adult rats was immersed in boiling water for either 3 sec (PTB) or 10 sec (FTB). In 10 control rats, the same area was immersed in room temperature water. Rats were sacrificed at either 3 or 48 hr after burn, and skin samples were analyzed to determine spin-lattice (T1) and spin-spin (T2) relaxation times. TWC was then measured gravimetrically by desiccation. Both T1 and T2 times significantly correlated with TWC (T1: r = 0.74, P < 0.0001; T2: r = 0.75; P < 0.0001). Both PTB and FTB resulted in significant elevations of T1, T2, and TWC 3 hr after injury (P > 0.001). At 48 hr postburn the T1, T2, and TWC of the PTB group had decreased to control values (P > 0.05), while all FTB parameters remained significantly elevated as compared to both the control and the 48-hr PTB parameters (P < 0.001). In vitro NMR measurements distinguish PTB from FTB in this rat model within 48 hr. These data provide a basis for investigating in vitro NMR techniques for the noninvasive assessment of burn wound depth.",
author = "Koruda, {Mark J.} and Andrea Zimbler and Settle, {R. Gregg} and Jacobs, {Danny O.} and Rolando Rolandelli and Wolf, {Gerald L.} and Rombeau, {John L.}",
year = "1986",
month = "1",
day = "1",
doi = "10.1016/0022-4804(86)90218-0",
language = "English (US)",
volume = "40",
pages = "475--481",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "5",

}

Koruda, MJ, Zimbler, A, Settle, RG, Jacobs, DO, Rolandelli, R, Wolf, GL & Rombeau, JL 1986, 'Assessing burn wound depth using in vitro nuclear magnetic resonance (NMR)', Journal of Surgical Research, vol. 40, no. 5, pp. 475-481. https://doi.org/10.1016/0022-4804(86)90218-0

Assessing burn wound depth using in vitro nuclear magnetic resonance (NMR). / Koruda, Mark J.; Zimbler, Andrea; Settle, R. Gregg; Jacobs, Danny O.; Rolandelli, Rolando; Wolf, Gerald L.; Rombeau, John L.

In: Journal of Surgical Research, Vol. 40, No. 5, 01.01.1986, p. 475-481.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Assessing burn wound depth using in vitro nuclear magnetic resonance (NMR)

AU - Koruda, Mark J.

AU - Zimbler, Andrea

AU - Settle, R. Gregg

AU - Jacobs, Danny O.

AU - Rolandelli, Rolando

AU - Wolf, Gerald L.

AU - Rombeau, John L.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - There is no accurate noninvasive method for differentiating between partial-thickness and full-thickness cutaneous burn wounds. Full-thickness burns (FTB) result in slower resorption of wound edema than partial thickness burns (PTB). Since proton NMR parameters, particularly the T1 relaxation time, correlate with tissue water content (TWC), the present study determined whether proton NMR could distinguish PTB from FTB. An area of upper dorsum (∼ 15% BSA) of 35 adult rats was immersed in boiling water for either 3 sec (PTB) or 10 sec (FTB). In 10 control rats, the same area was immersed in room temperature water. Rats were sacrificed at either 3 or 48 hr after burn, and skin samples were analyzed to determine spin-lattice (T1) and spin-spin (T2) relaxation times. TWC was then measured gravimetrically by desiccation. Both T1 and T2 times significantly correlated with TWC (T1: r = 0.74, P < 0.0001; T2: r = 0.75; P < 0.0001). Both PTB and FTB resulted in significant elevations of T1, T2, and TWC 3 hr after injury (P > 0.001). At 48 hr postburn the T1, T2, and TWC of the PTB group had decreased to control values (P > 0.05), while all FTB parameters remained significantly elevated as compared to both the control and the 48-hr PTB parameters (P < 0.001). In vitro NMR measurements distinguish PTB from FTB in this rat model within 48 hr. These data provide a basis for investigating in vitro NMR techniques for the noninvasive assessment of burn wound depth.

AB - There is no accurate noninvasive method for differentiating between partial-thickness and full-thickness cutaneous burn wounds. Full-thickness burns (FTB) result in slower resorption of wound edema than partial thickness burns (PTB). Since proton NMR parameters, particularly the T1 relaxation time, correlate with tissue water content (TWC), the present study determined whether proton NMR could distinguish PTB from FTB. An area of upper dorsum (∼ 15% BSA) of 35 adult rats was immersed in boiling water for either 3 sec (PTB) or 10 sec (FTB). In 10 control rats, the same area was immersed in room temperature water. Rats were sacrificed at either 3 or 48 hr after burn, and skin samples were analyzed to determine spin-lattice (T1) and spin-spin (T2) relaxation times. TWC was then measured gravimetrically by desiccation. Both T1 and T2 times significantly correlated with TWC (T1: r = 0.74, P < 0.0001; T2: r = 0.75; P < 0.0001). Both PTB and FTB resulted in significant elevations of T1, T2, and TWC 3 hr after injury (P > 0.001). At 48 hr postburn the T1, T2, and TWC of the PTB group had decreased to control values (P > 0.05), while all FTB parameters remained significantly elevated as compared to both the control and the 48-hr PTB parameters (P < 0.001). In vitro NMR measurements distinguish PTB from FTB in this rat model within 48 hr. These data provide a basis for investigating in vitro NMR techniques for the noninvasive assessment of burn wound depth.

UR - http://www.scopus.com/inward/record.url?scp=0022475401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022475401&partnerID=8YFLogxK

U2 - 10.1016/0022-4804(86)90218-0

DO - 10.1016/0022-4804(86)90218-0

M3 - Article

VL - 40

SP - 475

EP - 481

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 5

ER -