Analysis of autoantibody binding to 52‐kd paraneoplastic cerebellar degeneration–associated antigen expressed in recombinant proteins

Koichiro Sakai, Tomoko Ogasawara, Genjiro Hirose, Kurt Jaeckle, John E. Greenlee

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

A 52‐kd neural antigen was reported to be recognized by anti‐Purkinje cell antibodies in serum of a patient with paraneoplastic cerebellar degeneration associated with uterine carcinoma. In this study, we demonstrate that this neural antigen is recognized by antibodies known as anti‐Purkinje cell antibody type I (PCAb Type I) and anti‐YO. The latter's antigen is reported to be specific for the 62‐ to 64‐kd antigen CDR62. Assuming that the 52‐kd and 62‐ to 64‐kd antigens share a common epitope(s) recognized by all of these antibodies, we examined the antigenic region on the 52‐kd protein by immunoblots with deletion fragment proteins of the recombinant 52‐kd protein. A major epitope was localized in the region of amino acid residues 94 to 133 of the 52‐kd protein, which is the site of a leucine zipper motif. The potential pathogenicity of PCAb Type I is discussed.

Original languageEnglish (US)
Pages (from-to)373-380
Number of pages8
JournalAnnals of Neurology
Volume33
Issue number4
DOIs
StatePublished - Jan 1 1993

Fingerprint

Recombinant Proteins
Autoantibodies
Antigens
Antibodies
Epitopes
Paraneoplastic Cerebellar Degeneration
Leucine Zippers
Proteins
Virulence
Carcinoma
Amino Acids
Serum

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Sakai, Koichiro ; Ogasawara, Tomoko ; Hirose, Genjiro ; Jaeckle, Kurt ; Greenlee, John E. / Analysis of autoantibody binding to 52‐kd paraneoplastic cerebellar degeneration–associated antigen expressed in recombinant proteins. In: Annals of Neurology. 1993 ; Vol. 33, No. 4. pp. 373-380.
@article{cadf558249e848b79eb40c137faf098a,
title = "Analysis of autoantibody binding to 52‐kd paraneoplastic cerebellar degeneration–associated antigen expressed in recombinant proteins",
abstract = "A 52‐kd neural antigen was reported to be recognized by anti‐Purkinje cell antibodies in serum of a patient with paraneoplastic cerebellar degeneration associated with uterine carcinoma. In this study, we demonstrate that this neural antigen is recognized by antibodies known as anti‐Purkinje cell antibody type I (PCAb Type I) and anti‐YO. The latter's antigen is reported to be specific for the 62‐ to 64‐kd antigen CDR62. Assuming that the 52‐kd and 62‐ to 64‐kd antigens share a common epitope(s) recognized by all of these antibodies, we examined the antigenic region on the 52‐kd protein by immunoblots with deletion fragment proteins of the recombinant 52‐kd protein. A major epitope was localized in the region of amino acid residues 94 to 133 of the 52‐kd protein, which is the site of a leucine zipper motif. The potential pathogenicity of PCAb Type I is discussed.",
author = "Koichiro Sakai and Tomoko Ogasawara and Genjiro Hirose and Kurt Jaeckle and Greenlee, {John E.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1002/ana.410330407",
language = "English (US)",
volume = "33",
pages = "373--380",
journal = "Annals of Neurology",
issn = "0364-5134",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

Analysis of autoantibody binding to 52‐kd paraneoplastic cerebellar degeneration–associated antigen expressed in recombinant proteins. / Sakai, Koichiro; Ogasawara, Tomoko; Hirose, Genjiro; Jaeckle, Kurt; Greenlee, John E.

In: Annals of Neurology, Vol. 33, No. 4, 01.01.1993, p. 373-380.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of autoantibody binding to 52‐kd paraneoplastic cerebellar degeneration–associated antigen expressed in recombinant proteins

AU - Sakai, Koichiro

AU - Ogasawara, Tomoko

AU - Hirose, Genjiro

AU - Jaeckle, Kurt

AU - Greenlee, John E.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - A 52‐kd neural antigen was reported to be recognized by anti‐Purkinje cell antibodies in serum of a patient with paraneoplastic cerebellar degeneration associated with uterine carcinoma. In this study, we demonstrate that this neural antigen is recognized by antibodies known as anti‐Purkinje cell antibody type I (PCAb Type I) and anti‐YO. The latter's antigen is reported to be specific for the 62‐ to 64‐kd antigen CDR62. Assuming that the 52‐kd and 62‐ to 64‐kd antigens share a common epitope(s) recognized by all of these antibodies, we examined the antigenic region on the 52‐kd protein by immunoblots with deletion fragment proteins of the recombinant 52‐kd protein. A major epitope was localized in the region of amino acid residues 94 to 133 of the 52‐kd protein, which is the site of a leucine zipper motif. The potential pathogenicity of PCAb Type I is discussed.

AB - A 52‐kd neural antigen was reported to be recognized by anti‐Purkinje cell antibodies in serum of a patient with paraneoplastic cerebellar degeneration associated with uterine carcinoma. In this study, we demonstrate that this neural antigen is recognized by antibodies known as anti‐Purkinje cell antibody type I (PCAb Type I) and anti‐YO. The latter's antigen is reported to be specific for the 62‐ to 64‐kd antigen CDR62. Assuming that the 52‐kd and 62‐ to 64‐kd antigens share a common epitope(s) recognized by all of these antibodies, we examined the antigenic region on the 52‐kd protein by immunoblots with deletion fragment proteins of the recombinant 52‐kd protein. A major epitope was localized in the region of amino acid residues 94 to 133 of the 52‐kd protein, which is the site of a leucine zipper motif. The potential pathogenicity of PCAb Type I is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0027406898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027406898&partnerID=8YFLogxK

U2 - 10.1002/ana.410330407

DO - 10.1002/ana.410330407

M3 - Article

C2 - 8489208

AN - SCOPUS:0027406898

VL - 33

SP - 373

EP - 380

JO - Annals of Neurology

JF - Annals of Neurology

SN - 0364-5134

IS - 4

ER -